What defines your business's hours of operation in Zendesk?

The correct choice is schedules, as they specifically determine the business's hours of operation in Zendesk. Schedules allow administrators to define when their support team is available to address customer inquiries. This can include setting specific times for different days of the week, accommodating holidays, or even creating unique schedules for departments within the organization.

When properly configured, schedules enable automated responses to be aligned with the availability of the support team, ensuring customers receive appropriate support based on defined operating hours. This is crucial for managing expectations regarding response times and service levels.

The other options do not set the hours of operation. Prioritized statuses relate to how issues are ranked or prioritized, triggers automate actions based on certain conditions being met, and macros provide pre-defined responses for common queries but do not influence operational timeframes.